LOCUS       40924_38098        6670 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRS444ADH DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Mumberg D, Muller R, Funk M.
  TITLE     Yeast vectors for the controlled expression of heterologous proteins
            in different genetic backgrounds
  JOURNAL   Gene 156 (1), 119-122 (1995)
  PUBMED    7737504
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Ohto C, Muramatsu M, Obata S, Sakuradani E, Shimizu S.
  TITLE     Overexpression of the gene encoding HMG-CoA reductase in 
            Saccharomyces cerevisiae for production of prenyl alcohols
  JOURNAL   Appl. Microbiol. Biotechnol. 82 (5), 837-845 (2009)
  PUBMED    19083230
REFERENCE   3  (bases 1 to 6670)
  AUTHORS   Ohto C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2007) Contact:Chikara Ohto Toyota Motor 
            Corporation, Bio Research Laboratory, Future Project Division; 1, 
            Toyota-cho, Toyota, Aichi 471-8572, Japan
REFERENCE   4  (bases 1 to 6670)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1995"; volume: "156"; issue: "1"; pages: "119-122"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2009"; volume: "82"; issue: "5"; 
            pages: "837-845"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2007) Contact:Chikara Ohto Toyota Motor Corporation, Bio 
            Research Laboratory, Future Project Division; 1, Toyota-cho, Toyota,
            Aichi 471-8572, Japan"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        187..467
                     /label=TRP1 promoter
     CDS             468..1139
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(2160..3040)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     3377..3393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3403..3421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3440..3687)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3697..3713
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3747..3763)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3780..4180)
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        complement(4410..4428)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4449..4465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4473..4489)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4497..4527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4542..4563)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4851..5439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5613..6470)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6471..6575)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccct tatcgatgat aagctgtcaa agatgagaat taattccacg gactatagac
     1621 tatactagat actccgtcta ctgtacgata cacttccgct caggtccttg tcctttaacg
     1681 aggccttacc actcttttgt tactctattg atccagctca gcaaaggcag tgtgatctaa
     1741 gattctatct tcgcgatgta gtaaaactag ctagaccgag aaagagacta gaaatgcaaa
     1801 aggcacttct acaatggctg ccatcattat tatccgatgt gacgctgcag cttctcaatg
     1861 atattcgaat acgctttgag gagatacagc ctaatatccg acaaactgtt ttacagattt
     1921 acgatcgtac ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg
     1981 aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca
     2041 atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg
     2101 tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta
     2161 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     2221 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     2281 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga cgagagcgct
     2341 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc
     2401 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga
     2461 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa
     2521 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt
     2581 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac
     2641 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg
     2701 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc
     2761 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta
     2821 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta
     2881 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa
     2941 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga
     3001 gatacttttg agcaatgttt gtggaagcgg tattcgcaat gggaagctcc accccggttg
     3061 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatggcgaa cgtggcgaga
     3121 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3181 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat
     3241 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3301 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     3361 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     3421 ggcgaattgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca
     3481 aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
     3541 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
     3601 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
     3661 gtaagcgtga cataactaat tacatgatgc ggccctcgag gtcgacggta tcgataagct
     3721 tgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcgga
     3781 gttgattgta tgcttggtat agcttgaaat attgtgcaga aaaagaaaca aggaagaaag
     3841 ggaacgagaa caatgacgag gaaacaaaag attaataatt gcaggtctat ttatacttga
     3901 tagcaagaca gcaaactttt tttatttcaa attcaagtaa ctggaaggaa ggccgtatac
     3961 cgttgctcat tagagagtag tgtgcgtgaa tgaaggaagg aaaaagtttc gtgtgcttcg
     4021 agatacccct catcagctct ggaacaacga catctgttgg tgctgtcttt gtcgttaatt
     4081 ttttccttta gtgtcttcca tcattttttt gtcattgcgg atatggtgag acaacaacgg
     4141 gggagagaga aaagaaaaaa aaagaaaaga agttgcatgc gcctattatt acttcaatag
     4201 atggcaaatg gaaaaagggt agtgaaactt cgatatgatg atggctatca agtctagggc
     4261 tacagtatta gttcgttatg taccaccatc aatgaggcag tgtaattggt gtagtcttgt
     4321 ttagcccatt atgtcttgtc tggtatctgt tctattgtat atctcccctc cgccacctac
     4381 atgttaggga ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4441 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4501 ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca
     4561 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4621 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4681 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4741 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5281 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5581 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5641 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5701 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5761 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5821 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5881 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5941 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6001 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6061 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6121 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6181 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6241 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6301 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6361 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6421 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6601 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6661 ccctttcgtc
//
